S2 Genomics의 Singulator 100 system은 single cell research시 가장 까다로운 과정인 tissue dissociation 과정을 cross contamination 걱정 없이 single-use cartridge를 사용해 자동으로 처리해 주는 장비로, fresh tissue (fresh frozen or OCT tissue)를 single-use cartridge에 넣으면 single nuclei 혹은 single cell 을 최소 7분 혹은 protocol 에 따라 1시간 정도면 얻을 수 있습니다.
Downstream으로 10x genomics, BD, Dolomite bio 제품 모두 연결해서 사용하실 수 있습니다.
| Cells | Nuclei | |
| Tissue Type | Fresh | Fresh, Frozen, OCT |
| Process Time | 20-60 minutes | 7 minutes |
| Yield* | 10,000 to > 500,000/mg | 20,000 to > 600,000/mg |
| Viability | 70 – 95% | N/A |
| * Varies depending on tissue types | ||
현재까지 테스트가 완료된 tissue 종류 (Nuclei)
| Type | Tissue |
| Human | *Aorta *Brain (Adult, Infant, Fetal) *Breast Tumor *Cerebral Organoids *Colon (Normal, Polyp & Tumor) *Heart (Adult & Fetal) *Hemangioma *Intestine (Fetal) *Lung (Fetal) *Muscle (TA & SA) *Prostate (Normal & Tumor) *Retinal organoids (WT & Gene Knockout) *Spleen (Fetal) *Thymus (Fetal) *Vascular Abnormality (Arterial) *Vascular Abnormality (Lymphatic) |
| Mouse | Brain Colon (Normal & PDX Tumor) Heart Intestine *Kidney (Normal & Pre-cystic) Liver Lung Lymph Muscle Pancreatic PDX Tumor *Spinal Cord Spleen |
| Spiny Mouse (A. cahirinus) | *Kidney |
| Rat | Brain Kidney Liver Lung Spleen |
| Honeybee (A. mellifera) | *Thorax |
| Arabidopsis | *Whole Seedling *Root Tip |
| * Customer-Lab Demonstrated | |